Evaluation of Supplementation of Glucose and Egg Yolk on the Motility of Cyprinus Carpio and Cirrhinus Mrigala Spermatozoa Under Short Term Storage

By : C. Judith Betsy , J. Stephen Sampath Kumar

Page No: 143-155

Abstract
Fish spermatozoa are reported to have the ability to utilize external energy supplements like glucose, egg yolk, honey, milk, bovine serum albumin, etc. when used as co-cryoprotectants during cryopreservation. Milt from Cyprinus carpio and Cirrhinus mrigala was collected, diluted to 100 times with 0.85% physiological saline containing glucose, egg yolk powder and raw hen’s egg yolk as the experimental component. Glucose was tried at eight different concentrations, viz., 0.25, 0.5, 0.75, 1.0, 2.5, 5, 10 and 15% (w/v); Egg yolk powder at three concentrations (5, 10 and 15% w/v) and raw hen’s egg yolk powder at three concentrations (5, 10 and 15% w/v). Observations on the motility duration of the spermatozoa were made once in 3h for 6h. There were marked influences of the supplemented items on the motility duration when compared to non-supplemented milt kept as Control. The differences in the motility duration between treatments were statistically significant (P>0.05). Glucose at all concentrations below 1% and raw hen’s egg yolk at 5% concentration were found positively influencing the spermatozoa while other concentrations including the egg yolk powder used did not exhibit any visible influence.

Authors :
C. Judith Betsy and J. Stephen Sampath Kumar
Department of Inland Aquaculture, Fisheries College & Research Institute, Tuticorin-628 008, India.