Enhancing the Life and Potency of Cyprinus CarpioSpermatozoa during Cryopreservation by Glucose Supplementation

By : C. Judith Betsy , J. Stephen Sampath Kumar

Page No: 71-79

Abstract
The present study was conducted to find out the optimal concentration of glucose that will help to enhance the life and potency of spermatozoa. Cyprinus carpio spermatozoa were cryopreserved along with glucose as a co-cryoprotectant after 1:100 dilution with 0.85% physiological saline as extender and DMSO as cryoprotectant (85:15). The diluents contained glucose at four different concentrations, viz., 0.25% (T1 ), 0.5% (T2 ), 0.75% (T3 ) and 1% (T4 ). The diluted milt was equilibrated for 10 min at 5°C and loaded into 0.25 ml straws. The loaded straws were then frozen with LN2 vapour for 5 min and immersed in liquid nitrogen. Observations were made once in 7 days for 42 days on motility parameters based on which the duration, score, pattern and percentage were determined. There were significant differences in the motility duration between treatments and glucose at 0.25% (T1) concentration was found to support the cryopreserved spermatozoa better than the other concentrations and the difference in motility duration was statistically significant (P > 0.05).

Authors :
C. Judith Betsy and J. Stephen Sampath Kumar
Department of Inland Aquaculture, Fisheries College & Research Institute, Tuticorin, India – 628 008.