Effect of Different Hormones on Change in Level of Testosterone and Estradiol in Murrel Channa Striatus (Bloch)

By : M. Chandramani , T. Francis , Rajagopalsamy. C.B.T. , Jawahar P. , N. Jayakumar , A. Subburaj

Page No: 45-52

Abstract
The effect of different hormones (Kisspeptin, GnRH analogue, Pituitary extract) on change in level of testosterone in male and estradiol in female and comparative analysis of change in the level of testosterone and estradiol-17b with respect to different hormones injected were investigated. Testosterone in blood serum of control and hormones injected fish was measured using Enzyme Linked Immunosorbent Assay kit to assess the influence of these hormones on the serum steroid hormone profile. Among the three hormones injected, Kisspeptin showed significant impact of increase in the level of testosterone in males and estradiol in females. The other two hormones showed less effect on steroid level of blood serum of male and female Channa striatus (Bloch). Among three hormones, Kisspeptin injected fish showed highest testosterone level of 4.1 ng/ml of blood during May and highest estradiol level of 9270 pg/ml of blood during April were observed. Among the three hormones injected Kisspeptin injected fish showed high impact on change in level of steroid hormones of murrel fish than GnRH analogue, Pituitary extract injected fish.

Authors :
M. Chandramani, T. Francis, P. Jawahar, N. Jayakumar And A. Subburaj
Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi - 628 008, Tamil Nadu, India.

C.B.T. Rajagopalsamy
Department of Inland Aquaculture, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi - 628 008, Tamil Nadu, India.