Effect of Ovarian Fluid on the Sperm Motility characteristic of Common Carp (Cyprinus Carpio)

By : C. Judith Betsy , J. Stephen Sampath Kumar

Page No: 225-231

Abstract
The composition of ovarian fluid of common carp, Cyprinus carpio Linnaeus, 1758 and their effects on sperm motility characteristics are not available. Based on the works available for other species in respect to ovarian fluid, the present study was carried out. The ovarian fluid of common carp was collected and evaluated for its pH, magnesium and calcium composition. The pH of ovarian fluid was in the range of 8. The calcium and magnesium content were in the range of 0.53±0.98 mM.L-1 and 0.62±0.48 mM.L-1 respectively. The sperm was activated using ovarian fluid, water and ovarian fluid + water with diluted and undiluted milt. When ovarian fluid alone was used, the sperm was not activated. However other two treatments like water and ovarian fluid + water activated the spermatozoa immediately. When activated with ovarian fluid + water, higher motility duration and percentage of motile spermatozoa was noticed and the results were statistically significant (P< 0.01).

Authors :
C. Judith Betsy
Department of Inland Aquaculture, Fisheries College & Research Institute, Tuticorin, India – 628 008.

J. Stephen Sampath Kumar
Centre for Sustainable Aquaculture, Tamil Nadu Fisheries University, Nagapattinam, India.