Dna Barcode Data Base Generation for Dotted Loach, Nemacheilus Semiarmatus (Day, 1867)

By : Jaculine Pereira. J , B. Ahilan , Jeya Shakila. R , Karal Marx. K , Rajagopalsamy. C.B.T. , Devivaraprasad Reddy

Page No: 115-125

Abstract
The Dotted loach, Nemacheilus semiarmatus was collected from the streams of Harangi river, Kalur village, Madikeri District of Karnataka state. Morphometric and meristic characterization was carried out for N. semiarmatus. The DNA was extracted from N. semiarmatus with phenol chloroform method. Quality of DNA was analyzed with UV spectrophotometer (260 nm and 280nm) and DNA was confirmed with Agarose (1%) gel Electrophoresis. DNA barcoding was carried out for the DNA extracts using Cytochrome c Oxidase subunit 1(COI) gene. Polymerase Chain Reaction (PCR) was carried with COI gene primer and the PCR product size of 652bp was confirmed with AGE (2%) using DNA marker (100bp ladder) and the DNA content was analyzed with Biophotometer (Eppendorf). The purity and concentration of DNA was analyzed at 260nm and 280 nm, the purity and concentration recorded for the PCR products were 1.82-1.89 (DNA quality is good) and 7.12 (4.6-10.1) respectively. PCR amplified products of COI gene were sequenced and submitted to Genbank (NCBI), the accession numbers were provided by GenBank for N. semiarmatus includes KP823400, KP823401 and KP823402.

Authors :
Jaculine Pereira. J, B. Ahilan, R. Jeya Shakila., K. Karal Marx, C.B.T.Rajagopalsamy and A. Devivaraprasad Reddy
Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi-628 008, Tamil Nadu.