Quality Assessment of Cryopreserved Sperm of Mrigal, Cirrhinus Cirrhosus and its use in Commercial Seed Production

By : Md. Arif Hossain , Md. Abdur Rouf , Naznin Akter , Md. Rafiqul Islam Sarder , Mohammad Matiur Rahman , Md. Fazlul Awal Mollah

Page No: 187-200

Abstract
Quality of frozen sperm of C. cirrhosus in terms of motility, fertility and hatching of eggs was examined in relation to extenders, cryoprotectants and storage time. Sperm was processed with Alsever’s solution, egg-yolk citrate or urea egg-yolk plus DMSO or methanol and highest post-thaw motility was observed from Alsever’s solution with 10% DMSO (91.6±.5%). In the first 4 weeks of cryostorage, sperm motility was similar (96.3±0.58% to 89.3±1.0%) to those of fresh (97.3±0.5%) whereas after 8 weeks of storage the motility reduced significantly (71.6±1.5%) (P<0.05). Fertilization of eggs inseminated with 4 and 8 weeks old sperm were 69.6±3.2% to 64.6±1.5%, close to that of fresh sperm (72.6±3.7% to 67.0±2.6) respectively. Hatching rates of cryopreserved sperm-originated larvae (60.3±2.5% to 52.6±2.5%) were also similar to that of fresh sperm (62.3±4.0% to 55.6±2.0%). The quality parameters of cryopreserved sperm of C. cirrhosus are standardized which measures the efficiency of cryopreservation protocol that can be used in seed production in hatcheries.

Authors :
Md. Arif Hossain, Md. Abdur Rouf, Naznin Akter, Md. Rafiqul Islam Sarder, Mohammad Matiur Rahman, and Md. Fazlul Awal Mollah
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh.
 

DOI: http://doi.org/10.32381/JAT.2018.33.3-4.7