Generation of DNA Barcode Data Base for Batik Loach, Nemacheilus Triangularis (Day)

By : Jaculine Pereira. J , B. Ahilan , Jeya Shakila. R , Karal Marx. K , Rajagopalsamy. C.B.T.

Page No: 173-185

Abstract
The Batik loach, Nemacheilus triangularis was collected from Karnataka state. Morphometric and meristic characterization was carried out for N. triangularis and DNA was isolated from N. triangularis using phenol chloroform rapid non-invasive technique. Purity of DNA was checked with UV spectrophotometer and DNA was confirmed with Agarose gel Electrophoresis. DNA barcoding was carried out with Polymerase Chain Reaction (PCR) of Cytochrome c Oxidase subunit 1(COI) gene and the PCR product 652bp was confirmed again with Agarose gel electrophoresis using DNA marker and DNA content was analysed with Biophotometer. Purity of DNA was ascertained and the purity value obtained was 1.60 -2.0 (DNA quality is good). Then, PCR products of COI gene were sequenced and submitted to Genbank (NCBI), the accession numbers were provided by GenBank for N. triangularis includes KP772689, KP772690 and KP772691.

Authors :
Jaculine Pereira. J
Department of Fisheries Biotechnology, Fisheries College and Research Institute, Thoothukudi – 628 008

Ahilan. B
Dr. MGR Fisheries College and Research Institute, Ponneri.

Jeya Shakila. R
Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi

Karal Marx. K
Institute of Fisheries Post Graduate Studies, Vanianchavadi, Chennai

Rajagopalsamy. C.B.T.
Aquatic Rainbow Technology Park, Madhavaram.
 

DOI: http://doi.org/10.32381/JAT.2018.33.3-4.6