The Journal of Plant Science Research - A UGC Care-Listed Journal

Published in Association with Forum For the Promotion of Plant Science Research

Current Volume: 39 (2023 )

ISSN: 0970-2539

e-ISSN: 0976-3880

Periodicity: Tri-annual

Month(s) of Publication: April, August & December

Subject: Botany

DOI: 10.32381/JPSR

Online Access is Free for Life Member

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The Journal of Plant Science Research is a reputed peer reviewed International Journal which is published Tri-Annual. This Journal disseminates knowledge in all related fields of Plant Science Research such as Plant Physiology, Agriculture, Bio-Chemistry and Botany. It imparts the latest advances in the field of biotechnology and genetic engineering which is proved beneficial for the upcoming Geneticists, Plant Physiologists, Botanists, Biochemists and Biotechnologists. It regularly supplies the latest information on researchers, education, publication, and projects on Plant Science at national and international levels.

A UGC Care-Listed Journal
National Academy of Agricultural Sciences (NAAS)
EBSCO
ProQuest
CABI
Genamics (JournalSeek)
Indian Citation Index (ICI)

 

Editor-In-Chief
Prof. Ashwani Kumar

University of Rajasthan,
Jaipur


President
Dr. P. C. Trivedi

Formerly Vice-Chancellor,
Jai Narain Vyas University, Jodhpur
M.D.S. University, Ajmer
DDU Gorakhpur University, Gorakhpur
Dr. R.M.L. Avadh University, Faizabad
Maharaja Ganga Singh University, Bikaner, Rajasthan
Former Head, Botany and Biotechnology, 
University of Rajasthan, Jaipur


Vice President
Dr. Rakesh Pandey

CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP)
Lucknow


Founder Chief Editor
Late Prof. C P Malik

FNA, Noida


Editorial Board
Dr. Krishnendu Mukherjee

Senior Scientist, Nuenster 48149, 
Germany


Prof. Shinjiro Ogita

Prefectural, University of Hiroshima
Hiroshima, Japan


Prof. Yuan-Yeu Yau

Northeastern State University,
Broken Arrow,
Oklahoma, USA


Advisors
Prof. H N Verma

Pro-Chancellor,
JNU, Jaipur


Volume 39 Issue 3 , (Jul-2023 to Sep-2023)

Editorial

By: ..

Page No : i

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Induced Morphological Viable Mutations in Mung Bean (Vigna radiata L. Millisp.)

By: Asha Rani

Page No : 1-7

Abstract
To find the viable morphological mutations in mung bean seeds of two varieties of green gram (Vigna radiata) i.e. Narendra mung-1 (NM-1) and Pantnagar mung-2 (PTM-2) were obtained from Pantnagar seed department, Pantnagar University, Pantnagar. These mutations occur due to the toxic effects of heavy metals which were present in city waste water. This effluent was collected from the B.D.A. colony nalah situated near Gulabrai Inter college of Bareilly city. The concentrations of the heavy metals such as copper, zinc and lead were found 7.500 mg/l, 7.270 mg/l and 3.740 mg/l respectively in city waste water. Different concentrations (80%, 90% and 100%) of the effluent were prepared to treat the mung bean seeds.
In first (M1) generation the phytotoxic effects of effluent on different growth parameters were observed. The seeds of M1 plants were collected and sown on plant to row progeny basis to raise second or M2 generation. Different type of viable morphological mutations were recorded in second (M2) generation. A wide range of viable mutants on seedling length, appearance, leaf number, shape and size, maturity pattern , yield were isolated in the M2 generation. Synchronous, early maturing and high yielding mutants are agronomically desirable which may be utilized in future breeding programme. Spectrum and frequency of these viable mutants were calculated. It has been observed that frequency of viable mutation increased with increasing concentrations of heavy metals. The heavy metal treatment showed increase in induction of viable mutation frequency and it reached to 3.255 in 100% ZnSO4 in PTM-2.

Author :
Asha Rani : Ph. D, Department of Botany, Bareilly College, Bareilly, M.J.P. Rohilkhand University, Bareilly, Uttar Pradesh, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.1

Price: 251

Depiction and Annotation of Hypothetical Proteins from Oryza sativa L. by Relative Screening in Seek of Rare and Nifty Proteins

By: Arpita Banerjee , Monali Bhattacharjee , Debapriya Das , Renia Mullick , Sujata Dhar (Dutta) , Debleena Roy

Page No : 9-19

Abstract
Rice (Oryza sativa L.) of the family Poaceae has various genomes that are uncharacterized for their biochemical, biophysical, and/or cellular functions, are identified as Hypothetical Proteins (HPs). In this study, a comparative in silico pipeline has been used for the identification and functional annotation of 10HPs of O. sativa obtained from NCBI database. The structure, function, subcellular localization and interacting partners of the proteins were analysed along with orthology prediction to the hypothetical proteins of the data set. 90% successful annotation was done where most of the annotated HPs have functionally significant domains and protein superfamilies. Majority of the proteins were predicted to be Enzymes. Subcellular localization is a vital step for effective protein identification as subcellular localization of HPs elucidates their cellular mechanism. Most of the proteins were localized within the nucleus followed by cytoplasm, chloroplast, mitochondria, plasma membrane, extracellular matrix. No signal peptide was predicted. Mainly, the proteins had their closest orthologous members belonging to other species of Oryza. Functional analysis has revealed the role of few HPs in both biotic and abiotic stress management in the plants. The consequence of this research may be beneficial for outlining general set pipeline or etiquettes for an improved perception of the function of HPs in physiological development of several plant system. The understanding of the structure and function of these important proteins and their binding sites would be beneficial in understanding their role in metabolic pathway and in docking studies for aiding in the drug discovery.

Authors :
Arpita Banerjee, Monali Bhattacharjee, Debapriya Das, Renia Mullick and Debleena Roy : 
PG Department of Botany, Lady Brabourne College, Kolkata, India
Sujata Dhar (Dutta) : Department of Zoology, Lady Brabourne College, Kolkata, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.2

Price: 251

Proline Metabolism in Two High Yielding Groundnut Cultivars that Differ in their Drought Tolerance

By: K.V. Madhusudhan , C. Sudhakar

Page No : 21-29

Abstract
In this study, we compared the effects of drought stress on proline metabolism, glycine betaine and lipid peroxidation in two groundnut (Arachis hypogaea L.) cultivars, K-6 and JL-24, that are droughttolerant and drought-susceptible, respectively. As RWC decreased over time, leaf dry weight values also decreased across all stress regimes and cultivars. In the leaves of both cultivars, pyrroline-5- carboxylate reductase (P5CR) was upregulated in response to water stress, while proline oxidase (POX) and pyrroline-5-carboxylate dehydrogenase (P5CDH) were downregulated. These results imply that the induction of proline levels during groundnut development is associated with the elevation of proline synthesis enzyme (P5CR) and the downregulation of proline degrading enzymes (POX and P5CDH) that contribute to the plant’s decreased sensitivity to drought. Proline levels are greater in cultivar K-6 than in cultivar JL-24, which may be attributed to increased P5CR rates and less inhibition of POX and P5CDH. At all stress levels, the groundnut cv. K-6 accumulated more glycine betaine than the cv. JL-24. Malondialdehyde (MDA) is an indicator of lipid peroxidation, and in cultivar JL-24 it was found in significantly higher concentrations than in cultivar K-6. Dry mass buildup and physio-biochemical responses differed across cultivars and were based on the intensity of the stress. Tolerance levels of these two cultivars were compared.

Authors :
K.V. Madhusudhan : Government College for Men, Cluster University, Kurnool, AP, India.
C. Sudhakar : Department of Botany, S.K. University, Anatapuramu, AP, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.3

Price: 251

Starch-based on Electrospun Nanofibrous Scaffold Incorporated with Bioactive Compound from Mollugo nudicaulis for Wound Healing Application

By: Mani Panagal , Siva Shankar Raj

Page No : 31-41

Abstract
The aim of this research was to develop nanofibrous scaffolds using starch and incorporating 12-(10-carboxydecanoyloxy)-12-oxododecanoic acid (Compound-1) from the ethanolic extract of Mollugo nudicaulis. Compound-1 was added to starch solutions at different concentrations (0, 20, 30, and 40% v/v) and transformed into nanofibers through electrospinning. The resulting electrospun fibers were analyzed for their morphology, size distribution, thermal stability, FT-IR spectra and wound healing potential. The nanofibers loaded with compound-1 exhibited a uniform morphology and had average diameters ranging from 76 to 98 nm. Moreover, the compound-1 encapsulated within the nanofibers demonstrated higher thermal stability compared to the free compound-1. FT-IR analysis revealed interactions between the starch and compound-1. Notably, the nanofibers loaded with 40% compound-1 exhibited significantly enhanced viability in HaCat cells. Western blot analysis showed increased expression levels of VEGF and PDGF proteins, suggesting that the developed nanofibers could promote angiogenesis and cell proliferation, thereby potentially accelerating the wound healing process. Based on these results, it can be concluded that the developed starch-based electrospun nanofibrous scaffolds, incorporating compound-1 from Mollugo nudicaulis, possess potent wound healing properties by activating the VEGF and PDGF signaling pathways. Nevertheless, further in vitro and in vivo studies are necessary to validate these findings.

Authors :
Mani Panagal and Siva Shankar Raj : Department of Biotechnology, Annai College of Arts and Science (Affiliated by Bharathidasan University), Kovilacheri, Kumbakonam, Tamil Nadu, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.4

Price: 251

Antioxidant and Acetylcholinesterase Inhibitory Activity of Basella alba Leaves

By: V.H. Patel , Vimal B. Jayswal , Neeta R. Dave

Page No : 43-48

Factors such as oxidative stress and reduced acetylcholine level have been implicated in Alzheimer’s disease. Green leafy vegetables exhibit neuroprotective activities by various mechanism, one of them being inhibition of acetylcholineesterase (AChE). Inhibition of AChE activity has been accepted as an effective treatment for neurodegenerative disease. AChE inhibitory activity prevents it from breaking down acetylcholine in the brain and improves acetylcholine concentration which leads to increased communication between the nerve cells. Some medicinal plants like Basella alba leaves (BAL) have been found to contain numerous phytochemicals with potent antioxidant effects. The majority of these component phytochemicals scavenge free radicals thereby neutralizing their injurious potentials on body tissues. BAL is an edible perennial vine found in tropical Asia and Africa, known as vegetables containing mucilage. The objective of this research is to estimate antioxidant capacity and AChE inhibitory activity of BAL. The total phenolic compounds, antioxidant capacity (FRAP, DPPH, ABTS, RPA) and AChE inhibitory activity of BAL were studied which results into total phenolic and flavonoid content of dried BAL was 664.49 mg GAE/ 100 gm and 950.52 mg RE/ 100 gm whereas total antioxidant capacity using FRAP, RPA, DPPH and ABTS was found to be 695.58, 429.17, 204.50 and 27.08 mg TE/ 100 gm, respectively. IC50 of fresh BAL for AChE inhibitory activity was 20.02 mg while that of positive control (tacrine) was found to be 0.008 mg. BAL have good antioxidant capacity and AChE inhibitory activity.

Authors :
Vimal B. Jayswal, V. H. Patel and Neeta R. Dave : P. G. Department of Home Science, Vallabh Vidyanagar, Anand, Gujarat, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.5

Price: 251

Selection of Candidate Plus Trees (CPTs) of Azadirachta indica from Different Provenances of South Eastern Rajasthan for Tree Improvement

By: Neetha. P , Subodh Dhonde

Page No : 49-62

Abstract
The present investigation was carried out in three districts of South Eastern Rajasthan; Jhalawar, Kota and Baran to select the phenotypically superior trees (CPTs) of Azadirachta indica and to study the variation of the phenotypic characters among provenances. Thirty CPTs each were selected for study from each of the three districts and the parameters tree height, crown diameter, bark thickness, Girth, clear bole height, Ratio of clear bole height and tree height were recorded and subjected to descriptive statistical analysis. The data analyzed for the CPTs selected reveals that both the Kota and Baran region have highest value for tree height, crown diameter, bark thickness, Girth, clear bole height, Ratio of clear bole height and tree height. The trees selected from Kota region is also having highest value of both crown diameter and second highest value of leaf area after Jhalawar region and the trees selected from Jhalawar region has the highest value for both Girth and bark thickness among other provenances. The data of this experiment can also be used as a base line data for using in further tree breeding and tree improvement programme of Azadirachta indica.

Authors :
Neetha. P : Assistant Professor, Department of Forest Biology and Tree Improvement, CH & F, Jhalawar, Agriculture University, Kota, Rajasthan, India.
Subodh Dhonde : Ph.D. Scholar, Forest Research Institute Deemed University, Dehradun, Uttrakhand, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.6

Price: 251

Flora of Series Inferae of Deoli Tehsil, Tonk District (Rajasthan)

By: O. P. Sharma

Page No : 63-67

Abstract
The paper highlights flora of series Inferae of Deoli tehsil (Tonk district) Rajasthan. The study is aimed to evaluate the taxonomic status of this series of the area. The observation is dependent on continuous collection tours of study area. Sixty-two plant species of series Inferae have been observed in this study.

Author :
O. P. Sharma : Professor, Head Department of Botany, Government College, Bundi, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.7

Price: 251

Documentation of Riparian Vegetation Across the Tungabhadra River, Vijayanagara District, Karnataka

By: D K Basavaraj , Raghavendra H , Mahesh Kammar , M. Siddeshwari , P Shivakumar Singh

Page No : 69-83

Abstract
The Thungabhadra river located on Vijayanagara district which having more Riparian vegetation comparatively other regions, the study reveals the majority of vegetation shows that among 83 species the dominant family is Fabaceae which is having 14 species (37%), 11 genus (15.4%) followed by Amaranthaceae 7 species (8.64%), 3 genus (4.22%) along with Asteraceae having 7 species (8.64 %) 7 genus (9.85 %). However, the present study finds that most of the commercially valuable, medicinal, and industrially enriched diverse species can be identified.

Authors :
D K Basavaraj, Raghavendra H, Mahesh Kammar and M. Siddeshwari : Department of PG Studies in Botany, Raichur University, Raichur, Karnataka, India
P Shivakumar Singh : Department of Botany, Palamuru University, Mahabubnagar, Telangana, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.8

Price: 251

Phytochemical Properties of Paederia cruddasiana Prain: A Medicinal Vine Used in Healing of Bone Fractures in Folk Medicine of Assam

By: Pronab Mudoi , B. K. Konwar

Page No : 85-94

Abstract
Kolialota (Paederia cruddasiana Prain), a vine belonging of the family Rubiaceae often used by traditional healers in Assam, India for treating bone fractures with sound results. However, there is no systematic research on the phytochemicals as well as on anabolic actions in bone healing. The study was taken up to analyze the phytochemicals and their biochemical activities present in the leaves of Kolialota. Results clearly refer to the terpenoids and flavonoids, which were found to be abundantly present in the leaves giving significant antioxidant properties. Among the tested extracts, methanolic extract was found to contain higher amounts of total phenols, flavonoids and antioxidants. In addition, the leaf extract was found to contain the high amount of calcium (3993 µg/L ) conformed by scanning electron microscope and energy dispersive analysis. Further, gas chromatography and mass spectroscopic studies also confirmed the presence of certain phytochemicals such as lidocaine and 4 dehydroxy-n-tyramine that are associated with bone anabolic properties.

Authors :
Pronab Mudoi and B. K. Konwar : Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.9

Price: 251

Mineral Nutrients Assessment of Selected Underutilized Yam Plants from Hamirpur District, Himachal Pradesh

By: Ram Chand Bhatti , Chongtham Nirmala , Sabir Hussain , Anand Narain Singh

Page No : 95-105

Abstract
This study was conducted on five wild tuber plants named Dioscorea alata, Dioscorea pentaphylla, Dioscorea bulbifera, Dioscorea deltoidea and Dioscorea bellophylla belonging to the family Dioscoreaceae which are edible and are eaten by the local people of Hamirpur district Himachal Pradesh. Collected samples of the selected underutilized Yam plants from different regions of the study area were analyzed for their mineral nutrient content. Twenty-one elements were found: Aluminium, Barium, Bromine, Calcium, Chloride, Copper, Iron, Potassium, Magnesium, Manganese, Molybdenum, Sodium, Niobium (columbium-Cb), Nickel, Ruthenium, Rubidium, Sulphur, Silicon, Strontium, Titanium and Zinc. However, maximum K, Mg, S, Mn, Cu, and P were recorded in Dioscorea alata, Ca in Dioscorea bulbifera, Fe and Zn in D. pentaphyla. Based on the chemical evaluation, it was observed that tubers of D. alata, D. bulbifera, D. pentaphyla, D. deltoidea, and D. bellophylla might be suggested for commercial cultivation. However, minor information is available regarding its edible properties and nutritional requirements to the modem civilization. Present knowledge is not enough to generalize for all types of exploited plants available in the basket of the Himalayan region. However, it can be helpful to optimize more research to promote food security for humanity from starvation and malnutrition.

Authors :
Ram Chand Bhatti : Smt. Aruna Asaf Ali Govt. Post Graduate College, Kalka, Panchkula, Haryana, India.
Chongtham Nirmala : Department of Botany, Panjab University, Chandigarh, India.
Sabir Hussain and Anand Narain Singh : Soil Ecosystem and Restoration Ecology Lab, Department of Botany, Panjab University, Chandigarh, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.10

Price: 251

Anticancer Property of Alstonia scholaris Linn Leaf Extract in MCF -7 and MDAMB -231 Breast Cancer Cell Line

By: Dhanakodi Shanmugapriya , Gopalan Jayanthi , Ranga Anantha Sayanam Rajeswari

Page No : 107-117

Abstract
Breast cancer is the major risk factor for mortality globally. Medicinal plant extracts were investigated as a potential source for breast cancer therapy. The current work aims to assess the intracellular ROS production and apoptosis induction by Alstonia scholaris Linn (A. scholaris) leaf extract in breast cancer cell lines. Column chromatography was used to purify bioactive components from an A. scholaris Linn methanolic extract. Fraction with a significant concentration of phytochemicals was utilised for further investigation. The results of DCFH-DA staining revealed a substantial rise in intracellular ROS levels in A. scholaris Linn-treated cell lines. The dual staining technique (AO/EtBr) was used to examine apoptotic changes. Treatment with A. scholaris Linn substantially enhanced the rate of apoptosis in breast cancer cell lines. Furthermore, enhanced p53 gene expression, downregulation of Bcl-2 and BRAC1 genes, and reduced TNF-a and IFN-l concentrations in A. scholaris Linn treated cancer cells demonstrated its anticancer activity.

Authors :
Dhanakodi Shanmugapriya and Gopalan Jayanthi : 
Assistant Professor, Department of Biochemistry, Government Arts College for Women, Krishnagiri, Tamil Nadu, India
Ranga Anantha Sayanam Rajeswari : Department of Biochemistry, School of Allied Health Sciences, Vinayaka Mission’s Kirupananda Variyar Medical College and Hospitals Campus, Vinayaka Mission’s Research Foundation (Deemed to be University), Salem, Tamil Nadu, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.11

Price: 251

Diagnosis of Blight Diseases in Tea Leaves Using Hybrid Deep Learning Approach

By: V. Sujatha , Nirmala V , Simi Margarat G , Ravindrakumar Selvaraj

Page No : 119-128

Abstract
Identification of leaf disease in tea plant in early stage is important to increase the production. In research so far in literature several artificial and machine learning approaches are investigated. In this paper, a new deep learning method is proposed combining the properties of GLCM, CNN and RNN. The method was developed for the diagnosis of disease using three types of data of tea leaf samples. The complex features were extracted from leaf samples. The extracted features were feed to the RNN classifier to differentiate abnormal and normal cases. The Investigation for disease classification is undergone using different pretrained CNN models, reshaping and feature map setting is done. The model is trained with the help of the Plant Village Dataset consisting of 54,305 images comprising of 38 different classes of both unhealthy and healthy leaves. The disease classification accuracy achieved by the proposed architecture is up to 95.81% and various observations were made with different hyperparameters of the CNN architecture. The experiment results achieved are comparable with other existing techniques in literature.

Authors :
V. Sujatha : Department of ECE, Shree Sathyam College of Engineering and Technology, Sankari, Tamil Nadu, India
Nirmala. V : Department of ECE, Assistant Professor, M. Kumarasamy College of Engineering, Karur, Tamil Nadu, India
Simi Margarat G : Department of Computer Science and Engineering, New Prince Shri Bhavani College of Engineering and Technology, Chennai, India
Ravindrakumar Selvaraj : Associate Professor, Biomedical Engineering, KIT- KalaignarKarunanidhi Institute of Technology, Coimbatore, Tamil Nadu, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.12

Price: 251

An Effective Protocol for the Micro-propagation of Spiny Coriander (Eryngium foetidum L.)

By: Roshan K. V. Remesh , Jyothish Madambikattil Sasi , Biju Dharmapalan , Dhanya D.S

Page No : 129-135

Abstract
Eryngium foetidum L., a biennial herb, is extensively employed as a medicinal plant in many tropical countries. Its cultivation as a spice plant has gained prominence in India, Vietnam, Australia, and other geographical locations. Moreover, this botanical specimen exhibits a wide array of ethnomedicinal applications, encompassing the treatment of thermal injuries, otalgia, pyrexia, hypertensive disorders, gastrointestinal sluggishness, convulsive episodes, bronchial spasms, abdominal discomfort, helminthic infestations, reproductive impairments, snake bites, enteric disturbances, and malarial infections. The objective of the present study was to establish the optimal nutritional conditions required for shoot multiplication. To achieve this, axillary buds were employed as the explant, and various media and plant growth regulators were tested to determine the most effective regeneration method. Out of the various concentrations of Murashige and Skoog (MS) media and hormones that were tested, the most effective plant regeneration, with a success rate exceeding 95%, occurred on a half-strength MS medium enriched with 1.0 mg L-1 of benzyl adenine (BA) and 0.1 mg L-1 of indole-3-acetic acid (IAA), resulting in the development of 5 shoots per explant. Subsequently, these shoots generated in vitro were successfully established with roots (85–100% success), and they were then transplanted to an experimental field with an efficiency rate of 65%. Overall, this study provides an optimized medium for the micropropagation of E. foetidum.

Authors :
Roshan K. V. Remesh : Assistant Professor, Sree Narayana Arts and Science College, Kumarakom, Kottayam, Kerala, India.
Jyothish Madambikattil Sasi : CSIR-National Institute of Science Communication and Policy Research (NIScPR), 14, Satsang Vihar Marg, New Delhi, India
Biju Dharmapalan : School of Natural Sciences and Engineering, National Institute of Advanced Studies, Indian Institute of Science Campus, Bangalore, India.
Dhanya D.S.: Department of Botany, Sree Narayana College for Women, Kollam, Kerala, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.13

Price: 251

Study on Secondary Metabolites of Some Ethnomedicinally Important Plants of Murshidabad District, West Bengal, India

By: Asim Mandal , Sandip More , Mithun Biswas , Sutibra Narayan Mondal , Souhardya Ray , Supriti Ghosh

Page No : 137-143

Abstract
Plant kingdom have enormous chemicals contained in them. From the very ancient time till nowadays, people utilize these medicinal properties of plants, for the treatment of many diseases. Traditional medical sciences like Ayurveda, Unani, and traditional manuscripts provide us with the knowledge of how different plant parts are used for the treatment of various diseases. Ethnic groups and indigenous people of rural Murshidabad district and West Bengal use these ethnomedicinally important plants for treatment. The information about the importance of medicinal plants is transferred from generation to generation since ancient times. The aim is to screen out the different secondary metabolites present in each of the samples. Secondary metabolites are not primarily used during the life cycle of plants but used for their defense or other activities. These secondary metabolites have several beneficial aspects on human health. The secondary metabolites which are screened out mainly from the angiosperm families, which are abundant in Murshidabad district. Out of 25 species of angiosperms 10 species are encountered in the Murshidabad district, West Bengal, India. The plant samples are collected from the different localities and different parts are used, and extraction is done by organic solvents and distilled water. The extracted samples are then used for the screening of the secondary metabolites. Secondary metabolites screened out from these 10 species are Tannins, Phenolics, Alkaloids, and certain Glycosylated forms. These secondary metabolites have been reported to have various health benefits like antiseptic, analgesic, nervous system regulation, antioxidant properties, hypoglycemic, and immunomodulatory activities.

Authors :
Asim Mandal, Sandip More and Mithun Biswas : Assistant Professor, Department of Botany, Krishnath College, West Bengal, India
Sutibra Narayan Mondal, Souhardya Ray and Supriti Ghosh : Department of Botany, Murshidabad University, West Bengal, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.14

Price: 251

Exploring the Phytochemical and Pharmacological Properties of Stinging Nettle (Urtica dioica L.): A Wild Edible Plant of Chamba District, Himachal Pradesh

By: Sumit , Gulshan Kumar , Sumit Singh

Page No : 145-154

Abstract
Urtica dioica L., commonly known as ‘stinging nettle’ is a wild edible Himalayan plant species that belongs to the family Urticaceae. This perennial herb grows in some pockets of Chamba district at an average elevation of 1900 to 2800 m and is locally called as ‘Ain’. The plant is used as great source of vegetable, fodder and herbal medicine. U. dioica L. cause skin irritation and itching when touched due to chemical compounds like acetylcholine, 5-hydroxy tryptamine, formic acid and histamine in the glandular hairs present on leaves and stem of the plant. The main objective of this study is to provide comprehensive information about phytochemistry and pharmacological aspects of U. dioica L. to strengthen traditional knowledge of native communities. The literature survey on U. dioica L. has been compiled from various sources such as scientific database-Google Scholar, regional floras and other published research articles. Kingsdraw chemical structure editor software has been used to depict the chemical structure of various compounds present in U. dioica L. such as phytosterols, flavonoids, tannins, proteins, amino acids and fatty acids.

Authors :
Sumit : Department of Botany, Govt. College Chamba, Himachal Pradesh, India.
Gulshan Kumar : Department of Botany, Career Point University, Hamirpur, Himachal Pradesh, India
Sumit Singh : Agrotechnology and Rural Development Division, CSIR-North-East Institute of Science and Technology, Jorhat, Assam, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.15

Price: 251

Synthesis of Silver Nanoparticles Using Ocimum sanctum Leaf Extract and Applications in the Textile Industry

By: Vadiraj B Tangod , Namrata M

Page No : 155-162

Abstract
Silver nanoparticles are synthesised using green nanotechnology and environmentally friendly chemistry, with Ocimum sanctum (Krishna Tulsi) leaf extract serving as a reducing and capping agent. This technique enables us to eliminate the usage of harmful reducing reagents while maintaining eco-friendly, cost-effective, and capable large-scale production due to the plentiful supply of greener products. This would accelerate science and technology forward at an alarming rate in the production of agricultural and industrial products, superior and durable materials, novel therapies for chronic and hated diseases like cancer, and plenty of additional industrial applications. According to the findings of this study, AgNPs from Krishna Tulsi extract have a great potential for usage as an antibacterial agent in the medical textile, food, and cosmetic industries.
The stability of synthesised molecules is examined using several components, such as temperature, time, and aggregation. The characterization was further examined using UV-Visible Spectroscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.

Authors :
Vadiraj B Tangod : Associate Professor and Head of Department of Physics, Government First Grade College for Women’s Opposite to R N Shetty Stadium Office, Dharwad, Karnataka, India
Namrata M : 
Associate Professor and Head of Department of Fashion Technology, Government First Grade College for Women’s Opposite to R N Shetty Stadium Office, Dharwad, Karnataka, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.16

Price: 251

Leaf Epidermal Features and Ethnomedicinal Studies of Vernonia cinerea (L.) Less. from Semi-arid Regions of Barmer, Rajasthan, India

By: Vimala , Dinesh Hans

Page No : 163-167

Abstract
By virtue of its floral form and chemical makeup, the family Asteraceae is widely distribute in India. It is regarded as one of the most developed dicotyledonous family. In the Asteraceae family, the genus Vernonia has herbs and shrubs. In India, Vernonia cinerea (L.) Less. is a widely distributed species. The plant has a reputation as a folklore remedy in several traditional medical systems. This study’s goal was to learn more about Vernonia cinerea (L.) Less. leaf epidermal micromorphology. On the abaxial and adaxial surfaces of leaves, glandular and non-glandular trichome types as well as anomocytic stomatal types were identified as diagnostic markers by qualitative and quantitative analysis of epidermal properties. On both leaf surfaces, the epidermal cells were predominantly sinuous or polygonal in shape. The study’s objective is to assess the Vernonia cinerea (L.) Less. plant’s therapeutic benefits among the local indigenous communities in the semi-arid areas of Barmer, Rajasthan.

Authors :
Vimala : Manikya Lal Verma Government College, Bhilwara, Rajasthan, India
Dinesh Hans : Department of Botany, SRK Government PG College, Rajsamand, Rajasthan, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.17

Price: 251

Studies on Effect of Salinity Stress with FYM on Growth and Productivity of Euphorbia Antisyphilitica Zucc.

By: Ashwani Kumar , Shobha Johari

Page No : 169-173

Abstract
Majority of east-coast belt of India is characterized by saline-alkaline soils. This condition is more serious in desert of Rajasthan. Hence, studies were undertaken to find out effect of irrigation with different saline concentrations along with FYM (farmyard manure) on Euphorbia antisyphilitica. This plant is a shrub, much branched, almost leafless and grows upto a height of approximately one meter. It has been reported to grow well in desert of Mexico and is useful to mankind in many ways like for biofuels, petrochemicals and for traditional medicines and waxes. Parameters taken for study were fresh and dry biomass, sugar, chlorophylls and hexane extractables of aboveground and underground parts. Our main emphasis was to study biomass production in saline soils which is a part of desertic environment of Rajasthan and by growing E. antisyphilitcia arid conditions of Thar desert can be improved. Experiments were performed in pots. Different concentrations of saline water taken for irrigation were – 0.125% , 0.25% , 0.50%, 1.0% and 2.0%. Fresh biomass and hexane extractables of aboveground parts were found to have a positive effect of saline water irrigation along with farmyard manure. Other parameters were also increased with different concentrations of saline water.

Authors :
Shobha Johari : Professor, Department of Botany, L.B.S. Government College, Kotputli, Rajasthan, India.
Ashwani Kumar : Former Head, Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.18

Price: 251

Phytosociological Study on Tree Species Diversity of Tribal Areas of Bichhua Tehsil, Chhindwara, Madhya Pradesh, India

By: Babita Rana , Kavita Chahal , Naveen Kumar Chourasia , Megha Jha , Rajendra Prasad Yadav

Page No : 175-181

Abstract
Understanding and evaluating population structure and floristic vegetation characteristics are crucial steps to reveal information about the relationships between distinct species. In order to evaluate the density, frequency, and abundance of the tree of Bichhua Tehsil, district Chhindwara, Madhya Pradesh, India, a systematic sampling technique was utilized to gather vegetation data from the natural vegetation. According to the study, there are 27 widely varying tree species belonging to 18 families. The species with the highest Importance Value Index (35.36) was Tectona grandis L.f., followed by Diospyros melanoxylon Roxb. with a score of 23.77. The Shannon Diversity Index (H) comes out to be is 3.244 reflecting the diversity of species in a given community and evenness is 0.98427. Such research would aid in identifying various levels of traditionally valued natural systems and planning for their better management with the ultimate goal of preserving biodiversity.

Authors :
Kavita Chahal : Government Model Science College, Jabalpur, Madhya Pradesh, India
Naveen Kumar Chourasia : Department of Zoology, Government College, Bichhua, Chhindwara, M.P., India
Babita Rana : Department of Botany, Guru Nanak Khalsa College of Arts, Science and Commerce, Matunga, Mumbai, India.
Megha Jha : Department of Biotechnology, R&D, Pinnacle Biomedical Research Institute, Bhopal, M.P., India
Rajendra Prasad Yadav : Professor, Department of Geography, Government College, Bichhua, Chhindwara, M.P., India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.19

Price: 251

A Study on Auxins, Cytokinins and a Few Nutrients of a Brown Alga: Padina Pavonica

By: G. Subramanian , P. Ravi

Page No : 183-185

Abstract
Seaweeds and the compounds they produce can aid in and improve plant growth. Comparatively speaking, they are less expensive and simpler to use than other bioproducts. In this study, the brown alga Padina pavonica was shown to contain growth regulators and specific mineral compositions. In this work, growth regulators including auxins and cytokinins were examined. The macronutrients found in Padina pavonica were nitrogen, phosphorus, and potassium. The nitrogen content level was the highest (262.15 mg/g dry wt.) among the three macronutrients. The quantitative level of zinc was higher (125 ppm) than that of other micronutrients, such as Cu, Fe, Mn, and Zn.

Authors :
P. Ravi and G. Subramanian : Post Graduate and Researcher, Department of Botany, Arignar Anna Government Arts College, Namakkal, Tamil Nadu, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.20

Price: 251

Net Zero Roadmap: A Global Pathway to Keep the 1.5°C: Global Biofuels Alliance Led by India at G20

By: Ashwani Kumar

Page No : 187-198

Abstract
Climate change is long term fluctuation in temperature, precipitation, wind and all other aspects of Earth’s climate. The Intergovernmental Panel on Climate Change (IPCC), a UNEP body formed under WMO is the leading international body for the assessment of climate change. For the first time in human history, concentrations of carbon dioxide (CO2 ), the primary global warming pollutant, hit 400 parts per million (ppm) in our planet’s atmosphere. IPCC has prepared a report on climate change, land degradation, sustainable land management, food security and greenhouse gas fluxes in terrestrial ecosystems. Humanity will overshoot the Paris Climate Agreement’s 1.5°C ‘safe’ guardrail on average global temperature rise. The report suggests that if no significant policy changes are undertaken the world will be warm by 4 degrees Celsius, which is what scientists are nearly unanimously predicting will happen by the end of the century. Recently, during the G20 held in India a Global Biofuels Alliance (GBA), an India-led ‘field-to-fuel’ initiative to develop and deploy biofuels as a green pathway for energy transition, job creation and economic growth was initiated. Recent efforts have focused on the development of renewable alternatives to fossil fuels, and cellulosic biomass has great potential to contribute to the demand for liquid fuel. Algal biofuels are in the nascent stage of development. A review of the present situation shall be presented.

Author :
Ashwani Kumar : Alexander von Humboldt Fellow (Germany), Former Head of the Department of Botany and Biotechnology, University of Rajasthan, Jaipur, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.21

Price: 251

Pteris vittata L. In Vitro Morphogenesis: Unlocking the Potential for Phytoremediation Advancements

By: Ritu Jain

Page No : 199-202

Abstract
Pteris vittata L. has emerged as a prominent arsenic hyperaccumulator, holding immense potential for phytoremediation strategies aimed at mitigating arsenic contamination in the environment. in vitro culture of explants obtained from rhizome, crozier portion of juvenile leaf and fully expanded young leaf of Pteris vittata L on MS medium supplemented with different concentration of auxins and cytokinin of all combination studied. In Pteris vittata L. shoot is formed in 2, 4-D plus BAP and NAA plus BAP combinations. Through an in-depth examination of tissue culture techniques and growth regulator utilization in the context of Pteris vittata L., this paper aims to contribute to the ongoing discourse on sustainable and effective strategies for remediating arsenic-contaminated environments.

Author :
Ritu Jain : Associate Professor, Department of Botany, G. M. Momin Women’s College, Bhiwandi, Mumbai, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.22

Price: 251

Ceropegia Threatened and Less Known Genus of Rajasthan

By: Shikha Gupta , Amit Kotiya , Pooja Mangal , Yogita Solanki , Papita Choudhary

Page No : 203-215

Abstract
Ceropegia L. is a genus of tuberous plants and it belongs to family Apocynaceae that is comprised of 244 species worldwide. It is considered to be xerophytic because of its escape mechanism against the hot period by perennial tubers. Its tubers are edible and pharmacologically important mainly due to the presence of a pyridine alkaloid ‘Cerpegin’. Habitat destruction, over exploitation, anthropogenic activities and poor seed germination are major intimidations to the survival of this plant which have caused their decline in the wild and lead it to the endangered plant category list. This review provides comprehensive information on the distribution, taxonomy, phytochemistry, importance, IUCN status, and protocols for various in vitro regeneration approaches.

Authors :
Pooja Mangal, Yogita Solanki, Papita Choudhary, Shikha Gupta and Amit Kotiya : Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.23

Price: 251

Study on Quantitative Analysis of Two Important Medicinal Plants Gloriosa superba L. and Celastrus paniculatus Wild

By: Santosh Sharma

Page No : 217-223

Abstract
Both the evergreen climbing shrub Celastrus paniculatus Wild and the perennial plant Gloriosa superba L. are members of the family Colchicaceae and have substantial therapeutic significance. There is a species of Gloriosa superba L. that belongs to the family Colchicaceae. Both Ayurveda and Siddha, two ancient Indian medical practices, as well as Unani, place a significant emphasis on the importance of these two. The purpose of this study is to get a deeper understanding of the nootropic effects of C. paniculatus, in addition to its phytochemistry, traditional use, and other pharmacological properties. The species Gloriosa superba L. and Celastrus paniculatus Wild are great examples of the historical and contemporary pharmacological value of traditional medicinal herbs. Both of these species may be found in South America. More study is required since the medicinal uses of these plants are somewhat varied, and their phytochemical profiles are quite rich. The results of this research highlight the need of better integrating natural resources like these with contemporary medical practices.

Author :
Santosh Sharma : Assistant Professor, Rajesh Pilot Govt P.G. College, Lalsot, Rajasthan, India.
 

DOI : https://doi.org/10.32381/JPSR.2023.39.03.24

Price: 251